id
stringlengths
1
5
tokens
listlengths
2
320
ner_tags
listlengths
2
320
400
[ "nm23", "genes", "encode", "proteins", "that", "participate", "in", "tumor", "metastasis", "regulation", "and", "in", "various", "fundamental", "cellular", "processes", ",", "although", "their", "mechanisms", "of", "action", "are", "still", "unknown", "." ]
[ 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
401
[ "Although", "all", "nm23", "proteins", "contain", "nucleoside", "diphosphate", "(", "NDP", ")", "kinase", "activity", ",", "it", "has", "not", "been", "established", "that", "the", "enzyme", "activity", "mediated", "the", "various", "functions", "of", "nm23", "proteins", "." ]
[ 0, 0, 9, 10, 0, 9, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]
402
[ "In", "the", "present", "experiment", ",", "we", "examined", "the", "effect", "of", "nm23", "proteins", "on", "various", "differentiation", "induction", "systems", "of", "human", "leukemic", "cells", "including", "HL", "-", "60", ",", "U937", ",", "HEL", "/", "S", ",", "KU812F", ",", "K562", ",", "and", "HEL", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 5, 6, 6, 0, 5, 0, 5, 6, 6, 0, 5, 0, 5, 0, 0, 5, 0, 0 ]
403
[ "Native", "human", "erythrocyte", "NDP", "kinase", "protein", "inhibited", "the", "induction", "of", "erythroid", "differentiation", "of", "HEL", ",", "KU812", "and", "K562", "cells", ",", "but", "not", "the", "induction", "of", "monocytic", "or", "granulocytic", "differentiation", "of", "HL", "-", "60", ",", "U937", "and", "HEL", "/", "S", "cells", "." ]
[ 9, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0, 5, 6, 6, 0, 0 ]
404
[ "The", "erythroid", "differentiation", "of", "HEL", "cells", "was", "inhibited", "by", "recombinant", "human", "nm23", "-", "H1", ",", "-", "H2", ",", "mouse", "nm23", "-", "M1", ",", "and", "-", "M2", "proteins", "." ]
[ 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
405
[ "Moreover", ",", "both", "the", "mutant", "nm23", "-", "H2His", "protein", "and", "truncated", "nm23", "-", "H2", "protein", "containing", "N", "-", "terminal", "(", "1", "-", "60", ")", "peptide", ",", "which", "do", "not", "have", "NDP", "kinase", "activity", ",", "also", "inhibited", "erythroid", "differentiation", "of", "HEL", "cells", "." ]
[ 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 10, 10, 10, 10, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]
406
[ "These", "results", "suggest", "that", "(", "1", ")", "the", "differentiation", "inhibitory", "activity", "of", "I", "-", "factor", "/", "nm23", "protein", "is", "not", "restricted", "to", "monocytic", "differentiation", "of", "M1", "cells", ",", "(", "2", ")", "the", "inhibitory", "activity", "is", "exhibited", "without", "species", "specificity", ",", "and", "(", "3", ")", "the", "differentiation", "inhibitory", "activity", "of", "the", "nm23", "/", "NDP", "kinase", "protein", "is", "independent", "of", "its", "enzyme", "activity", "and", "requires", "the", "presence", "of", "N", "-", "terminal", "peptides", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
407
[ "Lipopolysaccharide", "-", "induced", "E", "-", "selectin", "expression", "requires", "continuous", "presence", "of", "LPS", "and", "is", "inhibited", "by", "bactericidal", "/", "permeability", "-", "increasing", "protein", "." ]
[ 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0 ]
408
[ "Endothelial", "cells", "stimulated", "by", "LPS", "express", "E", "-", "selectin", ",", "which", "plays", "an", "important", "role", "in", "mediating", "neutrophil", "adhesion", "during", "inflammation", "." ]
[ 7, 8, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0 ]
409
[ "E", "-", "selectin", "is", "induced", "within", "1", "-", "2", "h", ",", "peaks", "at", "4", "-", "6", "h", ",", "and", "gradually", "returns", "to", "basal", "level", "by", "24", "h", "." ]
[ 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
410
[ "rBPI21", ",", "a", "recombinant", "N", "-", "terminal", "fragment", "of", "human", "bactericidal", "/", "permeability", "-", "increasing", "protein", "(", "BPI", "),", "inhibited", "LPS", "-", "induced", "E", "-", "selectin", "expression", "when", "added", "at", "the", "same", "time", "as", ",", "and", "up", "to", "6", "h", "after", ",", "LPS", "." ]
[ 9, 0, 0, 9, 10, 10, 10, 10, 0, 9, 10, 10, 10, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
411
[ "Delayed", "administration", "of", "rBPI21", "also", "affected", "LPS", "-", "mediated", "activation", "of", "the", "nuclear", "factor", ",", "NF", "-", "kappa", "B", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 10, 10, 0 ]
412
[ "Two", "to", "4", "h", "following", "LPS", "addition", "to", "endothelial", "cells", ",", "when", "NF", "-", "kappa", "B", "was", "already", "activated", ",", "addition", "of", "rBPI21", "resulted", "in", "marked", "reduction", "of", "NF", "-", "kappa", "B", "detectable", "at", "4", "or", "6", "h", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0 ]
413
[ "These", "results", "indicate", "that", "endothelial", "activation", "requires", "continuous", "presence", "of", "LPS", ",", "and", "rBPI21", "acts", "to", "reverse", "LPS", "-", "mediated", "endothelial", "activation", "by", "interrupting", "the", "on", "-", "going", "LPS", "signal", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
414
[ "GM", "-", "CSF", "and", "IL", "-", "2", "share", "common", "control", "mechanisms", "in", "response", "to", "costimulatory", "signals", "in", "T", "cells", "." ]
[ 9, 10, 10, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
415
[ "Antigen", "complexed", "with", "major", "histocompatibility", "complex", "class", "I", "or", "II", "molecules", "on", "the", "surface", "of", "antigen", "presenting", "cells", "interacts", "with", "the", "T", "cell", "receptor", "(", "TCR", ")", "on", "the", "surface", "of", "T", "cells", "and", "initiates", "an", "activation", "cascade", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0 ]
416
[ "So", "called", "costimulatory", "signals", ",", "mediated", "by", "other", "cell", "surface", "interactions", "or", "soluble", "cytokines", "produced", "by", "antigen", "presenting", "cells", ",", "are", "also", "required", "for", "complete", "T", "cell", "activation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
417
[ "High", "levels", "of", "cytokine", "gene", "expression", "in", "T", "cells", "also", "required", "both", "TCR", "and", "costimulatory", "signals", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 7, 8, 0, 0, 0, 9, 0, 0, 0, 0 ]
418
[ "The", "granulocyte", "-", "macrophage", "colony", "-", "stimulating", "factor", "requires", "sequences", "in", "the", "promoter", "as", "well", "as", "a", "powerful", "enhancer", "located", "3kb", "upstream", "to", "respond", "to", "TCR", "-", "like", "signals", "." ]
[ 0, 9, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]
419
[ "These", "promoter", "and", "enhancer", "regions", "are", "mainly", "activated", "by", "the", "transcription", "factor", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NFAT", ")." ]
[ 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 9, 0 ]
420
[ "The", "activation", "of", "NFAT", "by", "TCR", "signals", "has", "been", "well", "described", "for", "interleukin", "-", "2", "(", "IL", "-", "2", ")", "and", "IL", "-", "4", "gene", "transcription", "in", "T", "cells", "." ]
[ 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
421
[ "Costimulatory", "signals", ",", "such", "as", "activation", "of", "the", "CD28", "cell", "surface", "molecule", "on", "T", "cells", ",", "lead", "to", "activation", "through", "a", "distinct", "region", "of", "the", "granulocyte", "-", "macrophage", "colony", "-", "stimulating", "factor", "(", "GM", "-", "CSF", ")", "promoter", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0 ]
422
[ "This", "region", "is", "termed", "the", "CK", "-", "1", "or", "CD28RE", "and", "appears", "to", "bind", "specific", "members", "of", "the", "NF", "-", "kappa", "B", "family", "of", "transcription", "factors", "." ]
[ 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 10, 0 ]
423
[ "Human", "T", "leukemia", "virus", "type", "1", "(", "HTLV", "-", "1", ")", "infects", "T", "cells", "and", "can", "lead", "to", "increase", "GM", "-", "CSF", "expression", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0 ]
424
[ "We", "have", "found", "that", "the", "HTLV", "-", "1", "transactivator", "protein", ",", "tax", ",", "acts", "as", "a", "costimulatory", "signal", "for", "GM", "-", "CSF", "and", "IL", "-", "2", "gene", "transcription", ",", "in", "that", "it", "can", "cooperate", "with", "TCR", "signals", "to", "mediate", "high", "level", "gene", "expression", "." ]
[ 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]
425
[ "Tax", "activates", "the", "GM", "-", "CSF", "promoter", "through", "the", "CK", "-", "1", "/", "CD28RE", "region", "and", "also", "activates", "nuclear", "factor", "-", "kappa", "B", "binding", "to", "this", "region", "." ]
[ 0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 2, 1, 2, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 0 ]
426
[ "However", ",", "other", "transcription", "factors", "or", "coactivators", "of", "NF", "-", "kappa", "B", "are", "required", "for", "tax", "activation", "but", "these", "remain", "to", "be", "identified", "." ]
[ 0, 0, 0, 9, 10, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]
427
[ "The", "CK", "-", "1", "/", "CD28RE", "of", "GM", "-", "CSF", "shows", "a", "high", "degree", "of", "similarity", "to", "the", "IL", "-", "2", "CD28RE", "and", "the", "IL", "-", "3", "gene", "also", "contains", "a", "related", "region", "." ]
[ 0, 1, 2, 2, 1, 2, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 1, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0 ]
428
[ "This", "observation", ",", "together", "with", "the", "fact", "that", "both", "GM", "-", "CSF", "and", "IL", "-", "2", "respond", "to", "TCR", "signals", "via", "NFAT", ",", "implies", "a", "high", "degree", "of", "conservation", "in", "the", "regulation", "of", "cytokine", "gene", "expression", "in", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 10, 10, 0, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 7, 8, 0 ]
429
[ "Danazol", "decreases", "transcription", "of", "estrogen", "receptor", "gene", "in", "human", "monocytes", "." ]
[ 0, 0, 0, 0, 1, 2, 2, 0, 7, 8, 0 ]
430
[ "1", ".", "Administration", "of", "danazol", "for", "over", "one", "month", "reduced", "the", "levels", "of", "estrogen", "receptor", "(", "ER", ")", "and", "its", "mRNA", "to", "approximately", "50", "and", "20", "%,", "respectively", "in", "monocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0 ]
431
[ "2", ".", "Danazol", "did", "not", "alter", "the", "degradation", "rate", "of", "ER", "mRNA", "in", "monocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 7, 0 ]
432
[ "3", ".", "Danazol", "decreased", "the", "transcription", "rate", "of", "ER", "gene", "to", "approximately", "50", "%", "in", "monocytes", "in", "a", "run", "-", "on", "assay", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0 ]
433
[ "4", ".", "Danazol", "may", "release", "estrogen", "predominance", "via", "the", "reduction", "of", "transcription", "for", "ER", "gene", ",", "which", "leads", "to", "the", "reduction", "of", "ER", "mRNA", "and", "ER", "expressions", "in", "monocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 9, 0, 0, 7, 0 ]
434
[ "Functional", "characterization", "of", "novel", "IL", "-", "2", "transcriptional", "inhibitors", "." ]
[ 0, 0, 0, 0, 9, 10, 10, 0, 0, 0 ]
435
[ "IL", "-", "2", "-", "mediated", "T", "cell", "proliferation", "is", "a", "critical", "early", "event", "in", "the", "inflammatory", "process", "." ]
[ 9, 10, 10, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
436
[ "Formation", "of", "the", "NFAT", "-", "1", "transcriptional", "complex", "on", "the", "IL", "-", "2", "promoter", "is", "essential", "for", "IL", "-", "2", "transcription", "." ]
[ 0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 1, 2, 2, 2, 0, 0, 0, 9, 10, 10, 0, 0 ]
437
[ "Using", "a", "cell", "line", "that", "is", "stably", "transfected", "with", "a", "trimer", "of", "the", "NFAT", "-", "1", "regulatory", "element", "linked", "to", "a", "lac", "-", "Z", "reporter", "gene", ",", "we", "screened", "for", "inhibitors", "of", "NFAT", "-", "1", "-", "mediated", "beta", "-", "galactosidase", "activity", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 10, 10, 0, 0 ]
438
[ "WIN", "61058", "and", "WIN", "53071", "were", "identified", "as", "microM", "inhibitors", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
439
[ "These", "compounds", "also", "inhibited", "beta", "-", "galactosidase", "mRNA", "levels", "." ]
[ 0, 0, 0, 0, 3, 4, 4, 4, 0, 0 ]
440
[ "Similar", "inhibition", "of", "NFAT", "-", "1", "-", "mediated", "gene", "expression", "was", "observed", "in", "a", "second", "cell", "line", ",", "which", "is", "stably", "transfected", "with", "NFAT", "-", "1", "regulatory", "elements", "linked", "to", "the", "reporter", "gene", "for", "sCD8", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 1, 2, 2, 2, 0 ]
441
[ "At", "10", "microM", ",", "both", "compounds", "inhibited", "IL", "-", "2", "mRNA", "and", "protein", "levels", "in", "the", "NFAT", "-", "1", "-", "linked", "lac", "-", "Z", "transfectants", ",", "and", "in", "human", "lymphocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 7, 8, 0 ]
442
[ "Both", "compounds", "inhibited", "the", "mixed", "lymphocyte", "reaction", ",", "and", "this", "inhibition", "was", "reversed", "by", "exogenous", "IL", "-", "2", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]
443
[ "WIN", "53071", "inhibited", "IL", "-", "2", "production", "induced", "in", "the", "calcium", "-", "dependent", "PMA", "and", "ionomycin", "pathway", "." ]
[ 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
444
[ "Conversely", ",", "calcium", "-", "independent", "anti", "-", "CD28", "Ab", "and", "PMA", "-", "induced", "IL", "-", "2", "production", "was", "resistant", "." ]
[ 0, 0, 9, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0 ]
445
[ "Both", "compounds", "altered", "the", "NFAT", "-", "1", "transcriptional", "complex", ",", "causing", "its", "retarded", "mobility", "on", "gels", "." ]
[ 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0 ]
446
[ "By", "these", "functional", "criteria", ",", "we", "believe", "we", "have", "identified", "two", "structurally", "distinct", ",", "novel", "inhibitors", "of", "NFAT", "-", "1", "-", "mediated", "transcription", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0 ]
447
[ "Transcriptional", "regulation", "of", "the", "vacuolar", "H", "(+)-", "ATPase", "B2", "subunit", "gene", "in", "differentiating", "THP", "-", "1", "cells", "." ]
[ 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 5, 6, 6, 6, 6, 0 ]
448
[ "Monocyte", "-", "macrophage", "differentiation", "was", "used", "as", "a", "model", "system", "for", "studying", "gene", "regulation", "of", "the", "human", "vacuolar", "H", "(+)-", "ATPase", "(", "V", "-", "ATPase", ")." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 10, 10, 0 ]
449
[ "We", "examined", "mRNA", "levels", "of", "various", "V", "-", "ATPase", "subunits", "during", "differentiation", "of", "both", "native", "monocytes", "and", "the", "cell", "line", "THP", "-", "1", ",", "and", "found", "that", "transcriptional", "and", "post", "-", "transcriptional", "mechanisms", "could", "account", "for", "increases", "in", "cell", "V", "-", "ATPase", "content", "." ]
[ 0, 0, 3, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 7, 8, 0, 0, 5, 6, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0 ]
450
[ "From", "nuclear", "runoff", "experiments", ",", "we", "found", "that", "one", "subunit", "in", "particular", ",", "the", "B2", "isoform", "(", "Mr", "=", "56", ",", "000", "),", "was", "amplified", "primarily", "by", "transcriptional", "means", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
451
[ "We", "have", "begun", "to", "examine", "the", "structure", "of", "the", "B2", "subunit", "promoter", "region", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]
452
[ "Isolation", "and", "sequencing", "of", "the", "first", "exon", "and", "5", "'-", "flanking", "region", "of", "this", "gene", "reveal", "a", "TATA", "-", "less", "promoter", "with", "a", "high", "G", "+", "C", "content", "." ]
[ 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0 ]
453
[ "Primer", "extension", "and", "ribonuclease", "protection", "analyses", "indicate", "a", "single", "major", "transcriptional", "start", "site", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]
454
[ "We", "transfected", "promoter", "-", "luciferase", "reporter", "plasmids", "into", "THP", "-", "1", "cells", "to", "define", "sequences", "that", "mediate", "transcriptional", "control", "during", "monocyte", "differentiation", "." ]
[ 0, 0, 1, 2, 2, 2, 2, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
455
[ "We", "found", "that", "sequences", "downstream", "from", "the", "transcriptional", "start", "site", "were", "sufficient", "to", "confer", "increased", "expression", "during", "THP", "-", "1", "differentiation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0 ]
456
[ "DNase", "I", "footprinting", "and", "sequence", "analysis", "revealed", "the", "existence", "of", "multiple", "AP2", "and", "Sp1", "binding", "sites", "in", "the", "5", "'-", "untranslated", "and", "proximal", "coding", "regions", "." ]
[ 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
457
[ "Synergy", "between", "signal", "transduction", "pathways", "is", "obligatory", "for", "expression", "of", "c", "-", "fos", "in", "B", "and", "T", "cell", "lines", ":", "implication", "for", "c", "-", "fos", "control", "via", "surface", "immunoglobulin", "and", "T", "cell", "antigen", "receptors", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 9, 10, 0, 9, 10, 10, 10, 0 ]
458
[ "Expression", "of", "the", "protooncogene", "c", "-", "fos", "is", "controlled", "by", "three", "main", "regulatory", "pathways", "involving", "kinase", "C", ",", "cAMP", ",", "and", "calcium", "." ]
[ 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0 ]
459
[ "Kinase", "C", "mediates", "its", "effects", "via", "phosphorylation", "of", "serum", "response", "factor", "(", "SRF", ")", "which", "interacts", "with", "the", "serum", "response", "element", "(", "SRE", ");", "cAMP", "and", "calcium", "mediate", "their", "effects", "via", "phosphorylation", "of", "CREB", "(", "cAMP", "regulatory", "element", "binding", "protein", ")", "presumably", "by", "activation", "of", "a", "protein", "kinase", "A", "or", "calmodulin", "-", "regulated", "kinase", "." ]
[ 9, 10, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 10, 10, 10, 0 ]
460
[ "We", "have", "examined", "the", "function", "of", "these", "elements", "in", "Burkitt", "'", "s", "lymphoma", "cells", "(", "Ramos", "and", "Daudi", ")", "as", "well", "as", "a", "T", "lymphocytic", "cell", "line", "(", "Jurkat", ")." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 5, 0, 5, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 0 ]
461
[ "We", "have", "found", "that", "stimulation", "of", "any", "one", "of", "these", "pathways", "alone", "has", "little", "or", "no", "effect", "on", "c", "-", "fos", "induction", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0 ]
462
[ "However", ",", "kinase", "C", "activation", "(", "PMA", "stimulation", ")", "combined", "with", "either", "cAMP", "(", "forskolin", "plus", "MIX", ")", "or", "calcium", "stimulation", "(", "ionophore", ")", "leads", "to", "greatly", "enhanced", "c", "-", "fos", "induction", "." ]
[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0 ]
463
[ "By", "contrast", ",", "cAMP", "in", "the", "presence", "of", "calcium", "shows", "no", "synergy", "in", "c", "-", "fos", "induction", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0 ]
464
[ "Okadaic", "acid", "augments", "PMA", "-", "as", "well", "as", "calcium", "-", "mediated", "activation", "of", "c", "-", "fos", ",", "and", "has", "little", "or", "no", "effect", "when", "combined", "with", "cAMP", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
465
[ "The", "main", "difference", "between", "Ramos", "(", "B", "cells", ")", "and", "Jurkat", "(", "T", "cells", ")", "in", "the", "regulation", "of", "c", "-", "fos", "is", "that", "cAMP", "plus", "calcium", "is", "strongly", "synergistic", "in", "Jurkat", "and", "is", "without", "effect", "in", "Ramos", "." ]
[ 0, 0, 0, 0, 5, 0, 7, 8, 0, 0, 5, 0, 7, 8, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 5, 0 ]
466
[ "Analysis", "of", "AP", "-", "1", "activity", "using", "gel", "mobility", "shift", "assays", "confirms", "that", "the", "requirements", "for", "synergy", "in", "c", "-", "fos", "mRNA", "induction", "are", "paralleled", "by", "requirements", "for", "synergy", "in", "induction", "of", "AP", "-", "1", "activity", "." ]
[ 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0 ]
467
[ "Signaling", "in", "B", "cells", "due", "to", "anti", "-", "Ig", "stimulation", "involves", "both", "kinase", "C", "activation", "and", "release", "of", "intracellular", "calcium", ",", "and", "results", "in", "c", "-", "fos", "mRNA", "induction", "." ]
[ 0, 0, 7, 8, 0, 0, 9, 10, 10, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0 ]
468
[ "Our", "results", "indicate", "that", "synergy", "between", "the", "kinase", "C", "activation", "and", "calcium", "is", "needed", "for", "efficient", "c", "-", "fos", "induction", "since", "neither", "of", "these", "two", "alone", "induces", "c", "-", "fos", "well", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0 ]
469
[ "That", "synergy", "of", "signaling", "pathways", "is", "relevant", "for", "the", "anti", "-", "Ig", "induction", "of", "c", "-", "fos", "is", "supported", "by", "the", "fact", "that", "cAMP", "-", "inducing", "agents", "and", "okadaic", "acid", "further", "enhance", "anti", "-", "Ig", "induction", "of", "c", "-", "fos", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 1, 2, 2, 0 ]
470
[ "These", "results", "suggest", "that", "cell", "-", "specific", "patterns", "of", "synergy", "are", "an", "essential", "feature", "for", "c", "-", "fos", "induction", "and", "may", "be", "relevant", "for", "c", "-", "fos", "control", "through", "B", "and", "T", "cell", "antigen", "receptors", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
471
[ "Multiple", "signals", "are", "required", "for", "function", "of", "the", "human", "granulocyte", "-", "macrophage", "colony", "-", "stimulating", "factor", "gene", "promoter", "in", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 7, 8, 0 ]
472
[ "The", "human", "granulocyte", "-", "macrophage", "CSF", "(", "GM", "-", "CSF", ")", "gene", "is", "expressed", "in", "T", "cells", "in", "response", "to", "TCR", "activation", "that", "can", "be", "mimicked", "by", "treatment", "of", "the", "cells", "with", "PMA", "and", "Ca2", "+", "ionophore", "." ]
[ 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 7, 8, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
473
[ "The", "gene", "contains", "a", "proximal", "functional", "promoter", "region", "(", "-", "620", "to", "+", "34", "),", "as", "well", "as", "a", "powerful", "enhancer", "located", "3", "kb", "upstream", ",", "both", "of", "which", "are", "involved", "in", "the", "response", "of", "the", "gene", "to", "TCR", "activation", "." ]
[ 0, 0, 0, 0, 1, 2, 2, 2, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]
474
[ "The", "proximal", "promoter", "contains", "a", "region", "termed", "CLEO", "(", "-", "54", "to", "-", "31", ")", "that", "consists", "of", "a", "purine", "-", "rich", "element", "abutting", "an", "activator", "protein", "-", "1", "(", "AP", "-", "1", ")-", "like", "site", ",", "as", "well", "as", "an", "upstream", "nuclear", "factor", "-", "kappa", "B", "(", "NF", "-", "kappa", "B", ")", "site", "(", "-", "85", "to", "-", "76", ")", "and", "a", "CK", "-", "1", "element", "(", "-", "101", "to", "-", "92", ")." ]
[ 0, 1, 2, 0, 0, 0, 0, 1, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 1, 2, 2, 2, 2, 0, 0, 0, 1, 2, 2, 2, 0, 1, 2, 2, 2, 2, 0 ]
475
[ "We", "show", "in", "this", "work", "that", "mutations", "in", "either", "the", "purine", "-", "rich", "region", "of", "the", "CLEO", "element", "or", "the", "NF", "-", "kappa", "B", "site", "result", "in", "reduced", "PMA", "/", "Ca2", "+", "activation", "of", "a", "620", "-", "bp", "human", "GM", "-", "CSF", "promoter", "-", "luciferase", "reporter", "construct", "in", "Jurkat", "T", "cells", "by", "65", "%", "and", "50", "%,", "respectively", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0 ]
476
[ "The", "major", "inducible", "protein", "complex", "that", "binds", "to", "the", "human", "CLEO", "(", "hCLEO", ")", "element", "is", "an", "AP", "-", "1", "-", "like", "complex", "that", "is", "inducible", "by", "PMA", "alone", ",", "but", "shows", "increased", "binding", "in", "response", "to", "PMA", "together", "with", "Ca2", "+", "ionophore", "." ]
[ 0, 0, 9, 10, 10, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 9, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
477
[ "Although", "the", "binding", "of", "this", "complex", "is", "not", "cyclosporin", "-", "sensitive", ",", "promoter", "induction", "is", "inhibited", "by", "cyclosporin", "treatment", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
478
[ "A", "second", "weak", "inducible", "complex", "resembling", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NF", "-", "AT", ")", "was", "also", "observed", "binding", "to", "the", "hCLEO", "region", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]
479
[ "By", "using", "recombinant", "proteins", ",", "we", "confirmed", "that", "AP", "-", "1", ",", "NF", "-", "ATp", ",", "and", "a", "higher", "order", "NF", "-", "ATp", "/", "AP", "-", "1", "complex", "could", "all", "form", "with", "the", "hCLEO", "element", ",", "and", "we", "have", "also", "defined", "the", "sequence", "requirements", "for", "binding", "of", "each", "of", "these", "complexes", "." ]
[ 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 10, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
480
[ "We", "found", "that", "expression", "of", "a", "constitutively", "active", "form", "of", "calcineurin", "could", "substitute", "for", "Ca2", "+", "ionophore", "and", "synergize", "with", "PMA", "to", "activate", "the", "GM", "-", "CSF", "promoter", ",", "and", "conversely", "that", "mutant", "-", "activated", "Ras", "could", "substitute", "for", "PMA", "and", "cooperate", "with", "Ca2", "+", "ionophore", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
481
[ "Co", "-", "expression", "of", "Ras", "and", "calcineurin", ",", "however", ",", "did", "not", "activate", "the", "GM", "-", "CSF", "promoter", ",", "but", "required", "the", "additional", "expression", "of", "NF", "-", "kappa", "B", "p65", "." ]
[ 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0 ]
482
[ "These", "results", "imply", "that", "at", "least", "three", "signals", "are", "required", "to", "activate", "the", "GM", "-", "CSF", "proximal", "promoter", ",", "and", "that", "the", "signals", "impinge", "on", "distinct", "transcription", "factors", "that", "bind", "to", "the", "hCLEO", "and", "NF", "-", "kappa", "B", "regions", "of", "the", "promoter", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 1, 0 ]
483
[ "IL", "-", "10", "induces", "the", "tyrosine", "phosphorylation", "of", "tyk2", "and", "Jak1", "and", "the", "differential", "assembly", "of", "STAT1", "alpha", "and", "STAT3", "complexes", "in", "human", "T", "cells", "and", "monocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 0, 7, 8, 8, 0, 7, 0 ]
484
[ "IL", "-", "10", "affects", "monocytes", "and", "T", "cells", "by", "driving", "the", "progression", "of", "immune", "responsiveness", "such", "that", "Th2", "lymphocyte", "-", "mediated", "effects", "predominate", "." ]
[ 9, 10, 10, 0, 7, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
485
[ "In", "this", "report", ",", "we", "show", "that", "in", "monocytes", "and", "T", "cells", "IL", "-", "10", "stimulates", "tyrosine", "phosphorylation", "of", "the", "signal", "transducers", "and", "activators", "of", "transcription", ",", "STAT1", "alpha", "and", "STAT3", ",", "in", "a", "differential", "manner", "such", "that", "the", "relative", "formation", "of", "homo", "-", "and", "hetero", "dimers", "varies", "between", "the", "two", "cell", "types", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 7, 8, 9, 10, 10, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 10, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
486
[ "Moreover", ",", "monocytes", "express", "a", "novel", "IL", "-", "10", "-", "stimulated", "STAT", "protein", "with", "an", "M", "(", "r", ")", "of", "70", "kDa", "that", "is", "recognized", "by", "the", "anti", "-", "STAT3", "Ab", "but", "is", "not", "observed", "in", "T", "cells", "." ]
[ 0, 0, 7, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 7, 8, 0 ]
487
[ "IL", "-", "10", "treatment", "of", "both", "T", "cells", "and", "monocytes", "results", "in", "the", "ligand", "-", "induced", "tyrosine", "phosphorylation", "of", "tyk2", "and", "Jak1", ",", "but", "not", "Jak2", "or", "Jak3", "." ]
[ 9, 10, 10, 0, 0, 0, 7, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 0, 9, 0 ]
488
[ "Selective", "modulation", "of", "immune", "responsiveness", "by", "IL", "-", "10", "in", "cells", "such", "as", "monocytes", "and", "T", "cells", "may", "result", "in", "part", "from", "the", "differential", "activation", "of", "STAT", "protein", "pairs", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 7, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]
489
[ "Use", "of", "new", "biologic", "markers", "in", "the", "ovulation", "induction", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
490
[ "Biological", "markers", "of", "ovulation", ",", "after", "a", "great", "in", "the", "past", ",", "have", "been", "fallen", "into", "disuse", "for", "the", "large", "diffusion", "of", "biochemical", "and", "biophysical", "ones", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
491
[ "However", ",", "the", "real", "effect", "of", "hormones", "involved", "in", "ovulation", "is", "expressed", "by", "biological", "modifications", "on", "target", "tissues", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
492
[ "To", "explore", "the", "modifications", "of", "not", "reproductive", "target", "tissues", "as", "ovulation", "markers", "we", "studied", "the", "behaviour", "of", "Albuminemia", ",", "Platelet", "Factor", "IV", "(", "as", "indicator", "of", "Platelet", "Aggregation", "),", "Type", "II", "estrogenic", "receptors", "in", "42", "ovulation", "induced", "women", ",", "undergoing", "our", "observation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
493
[ "33", "of", "them", "had", "ovulation", "and", "9", "developed", "a", "LUF", "syndrome", ",", "constituting", "two", "biological", "models", "of", "an", "opposite", "situation", "for", "the", "three", "markers", "observed", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
494
[ "All", "the", "markers", "considered", "were", "sufficiently", "sensitive", ",", "but", "among", "them", ",", "Platelet", "Factor", "IV", "was", "the", "most", "reliable", "to", "the", "hormonal", "ovulatory", "situation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
495
[ "Abnormal", "regulation", "of", "the", "IL", "-", "2", "promoter", "in", "lpr", "CD4", "-", "CD8", "-", "T", "lymphocytes", "results", "in", "constitutive", "expression", "of", "a", "novel", "nuclear", "factor", "of", "activated", "T", "cells", "-", "binding", "factor", "." ]
[ 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 10, 0 ]
496
[ "The", "inert", "quality", "of", "MRL", "-", "Ipr", "/", "Ipr", "(", "Ipr", ")", "peripheral", "CD4", "-", "CD8", "-", "(", "CD4", "-", "8", "-)", "T", "cells", "manifests", "primarily", "as", "an", "inability", "to", "proliferate", "or", "produce", "IL", "-", "2", "in", "response", "to", "TCR", "or", "mitogenic", "stimulation", "." ]
[ 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 9, 0, 0, 0, 0 ]
497
[ "Yet", "these", "same", "cells", "do", "initiate", "early", "TCR", "-", "mediated", "signaling", "events", ",", "such", "as", "generation", "of", "inositol", "phosphates", "and", "increased", "intracellular", "calcium", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
498
[ "They", "also", "display", "constitutively", "high", "levels", "of", "p59fyn", "and", "CD3", "zeta", "tyrosine", "phosphorylation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 0, 0, 0 ]
499
[ "The", "generation", "of", "second", "messengers", "in", "T", "cells", "normally", "leads", "to", "downstream", "signaling", "that", "results", "in", "transcriptional", "activation", "of", "the", "IL", "-", "2", "gene", "." ]
[ 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]